| Abstract:In order to investigate the function of PoKAS gene in the fatty acid synthesis of P. ostii ‘Feng Dan’, we obtained three PoKAS genes from the transcriptome data, cloned the genes and conducted the bioinformatics analysis. Their expressions were examined by qRT-PCR at 23, 45, 75, 100 and 125 days after flowering. The results showed that: (1) the full sequence length three cloned genes were 1 401, 1 692 and 1 215 bp, encoding 466, 563 and 404 aa, respectively. The analysis of the conserved domains revealed that they all contained the conserved domain of KAS and belonged to the superprotein family of cond-enzymes. (2) The phylogenetic tree analysis showed that they were clearly divided into three categories, indicating that they were relatively evolutionarily independent, named PoKASⅠ, PoKASⅡ and PoKASⅢ (GenBank accession numbers OP056413, OP056412, and OP056414, respectively). (3) The qRT-PCR analysis revealed, among the five periods of peony seed development after the flower drop, the expressions of PoKASⅠ and PoKASⅡ were significantly higher at 75 d and 45 d after flowering than those at other development periods; the expression of PoKASⅢ wassignificantly higher at 45-125 d after anthesis than that at 23 d after anthesis, indicating that PoKASⅢ could play an important role in the whole process of fatty acid synthesis in peony seeds, while PoKASⅡ mainly could play a role in the early stage of rapid accumulation of seed oil. It is hypothesized that this gene family from P. ostiis ‘Feng Dan’ could play different roles in different developmental periods during the formation of seed fatty acids. |
