杂交兰及其花艺突变体中类黄酮成分差异的代谢-转录组联合分析
DOI:
作者:
作者单位:

福建省农业科学院作物研究所

作者简介:

通讯作者:

中图分类号:

基金项目:

福建省自然科学基金(2022J01478);福建省农业科学院青年英才项目(YC20210040,YC2019001);‘5511’协同创新项目(XTCXGC2021016);福建省种业创新与产业化工程(ZYCX-LY-202102);特色花卉科技创新团队(CXTD2021010-2)


Intergrated Metabolomics and Transcriptomics Analysis Reveals Differential Flavonoids Compounds of Cymbidium Hybrid and Its Mutant
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    为探索杂交兰花艺突变体变异的分子机理,本研究以杂交兰‘玉凤’及其花艺突变体‘双艺金龙’为材料,利用靶向代谢组学和转录组学鉴定两者中类黄酮化合物含量差异及其相关通路上的差异表达基因。结果表明:(1)代谢组分析发现,共检测到38个差异代谢物,显著富集在类黄酮、黄酮和黄酮醇生物合成。在类黄酮生物合成途径上,根皮苷、黄腐醇、二氢山奈酚、二氢杨梅素和表没食子儿茶素含量升高,柚皮苷和二氢槲皮素含量降低;在黄酮和黄酮醇生物合成途径上,三叶豆苷和槲皮素含量均下降。(2)转录组分析发现,与类黄酮生物合成相关的差异基因(CCoAOMT、CHS、CHI、F3H和FLS)被显著富集。转录组数据中类黄酮生物合成相关基因的表达量与代谢物含量的变化趋势一致,表明类黄酮生物合成相关基因CHS、CHI、F3H和FLS低表达,促使下游代谢物合成受阻,导致‘双艺金龙’花艺的产生。本研究结果为深入研究杂交兰花艺形成的分子调控机制提供了参考信息。

    Abstract:

    In order to confirm the molecular basis underlying flower color variegation between Cymbidium hybrid ‘Yufeng’ and its mutant ‘Shangyijinlong’, targeted metabolomics and transcriptomics were used to identify the differentially expressed metabolites (DEMs) and differentially expressed genes (DEGs) involved in flavonoid biosynthesis. The results showed that: (1) by using targeted metabolomics techniques, we identi?ed 38 DEMs,which were significantly enriched in flavonoid biosynthesis, flavone and flavonol biosynthesis. In flavonoid biosynthesis pathway, the contents of phlorizin, xanthohumol, dihydrokaempferol, dihydromyricetin and epigallocatechin were all increased, the contents of naringin and dihydroquercetin were decreased. In addition, the contents of trifolin and quercetin were decreased in flavone and flavonol biosynthesis pathway. (2) Transcriptomics analyses indicated that ?avonoid biosynthesis-related DEGs (CCoAOMT, CHS, CHI, F3H and FLS) were found to be signi?cantly enriched. The results of the transcriptomics were in agreement with the components change of metabolites revealed by metabolomics, it indicated that the color change of ‘Shuangyijinlong’ might be attributed to the down-regulation of CHS、CHI、F3H and FLS that led to blocked of downstream metabolites synthesis. Our results provide reference information for further revealing the molecular regulatory mechanism of color change in Cymbidium hybrid.

    参考文献
    相似文献
    引证文献
引用本文
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2022-11-18
  • 最后修改日期:2023-03-02
  • 录用日期:2023-03-06
  • 在线发布日期:
  • 出版日期:

微信公众号二维码

手机版网站二维码