[Objective] The study aims to further study the leaf morphology and chlorophyll fluorescence characteristics of Actinidia arguta under greenhouse cultivation conditions, and to explore the difference of leaf photomorphogenesis between greenhouse and open field cultivation. [Methods] In this experiment, the 5-year-old plants of A. arguta varieties 'Jialv' and 'Kuilv' were used as test materials to determine the relative chlorophyll content and chlorophyll fluorescence parameters of leaves at different leaf ages under greenhouse and open field cultivation conditions. The differences in photomorphogenesis and chlorophyll fluorescence characteristics under greenhouse and open field cultivation conditions were analyzed. [Results] The photomorphogenesis of A. arguta cultivated in greenhouse and open field was between 1-40 d leaf age, and the fluorescence characteristics of the same variety under different cultivation conditions tended to be consistent. After flowering, the fluorescence characteristics of the same variety under different cultivation conditions were quite different. The leaf area of A. arguta cultivated in greenhouse was larger, the content of chlorophyll b was higher, and it tended to shade leaf characteristics. The performance parameters such as PIabs were significantly lower than those of open field cultivation. The absorption and capture ability of light energy was stronger, but the heat dissipation was higher. The energy used for electron transfer was lower than that of open field cultivation, and the chlorophyll fluorescence parameters showed adaptive changes to the environment. [Conclusion] The photomorphogenesis time of A. arguta leaves was 1-40 days after leaf expansion, which was equivalent to completion before flowering. The photomorphogenesis of A. arguta leaves in greenhouse cultivation was significantly different from that in open field cultivation, and the photosynthetic capacity decreased slightly with the increase of leaf area. Greenhouse cultivation changed the chlorophyll fluorescence characteristics of leaves to a certain extent and reduced the photosynthetic performance.