【Objective】 In order to improve TuMV resistance of non-forming Chinese cabbage, the interaction mechanism between BcCHC1 gene and TuMV was investigated. 【Methods】 In this study, a member of the clathrin heavy chain CHC gene family was identified from cabbage and a CHC1 was cloned, and subcellular localization of the CHC1 was performed. CI and 6K2 were screened from the candidate genes by bicolecular fluorescence complementary detection. VIGS induced BcCHC1 silencing and the plants died. BiFC test confirmed the interaction between BcCHC1 and TuMV proteins. 【Results】 The results showed that: (1) BcCHC1 gene was cloned successfully, and its coding sequence length was 5 124 bp, encoding 1 708 amino acids. (2) After 30 days of TuMV infection, the results of real-time fluorescence quantitative PCR showed that the relative expression of BcCHC1 in TuMV inoculated lines decreased significantly. (3) Subcellular localization found that BcCHC1 was located in the cell membrane and nucleus of tobacco epidermal cells. (4) The observation of BcCHC1 gene silenced lines showed that BcCHC1 silenced plants had died before TuMV inoculation. (5) Through BiFC experimental verification analysis, it was found that BcCHC1 could interact with CI and 6K2, and the interaction position with CI was mainly in the nucleus, while the interaction position with 6K2 was mainly in the cell membrane. 【Conclusion】 The study speculated that BcCHC1 interacts with TuMV''s CI and 6K2 to regulate TuMV infection of non-knotting Chinese cabbage by influencing clastrin-dependent endocytosis pathway and viral replication, etc. However, the specific mechanism of BcCHC1 regulating TuMV infection of non-knotting Chinese cabbage needs further study.