[Objective] The diurnal variation characteristics of photosynthesis and chlorophyll fluorescence of greenhouse grape varieties in Hotan area were discussed, and their photosynthetic capacity was comprehensively evaluated, so as to provide reference for the introduction and cultivation management measures of greenhouse grapes in this area. [Methods] Six grape varieties ‘Hutai 8’‘Queen Nina’‘Summer Black’‘Sunshine Muscat’‘Xinyu’ and ‘Crimson Seedless’ were introduced into Hotan greenhouse as experimental materials. The photosynthetic active radiation (PAR), relative chlorophyll content (SPAD), photosynthesis and chlorophyll fluorescence parameters of each variety were measured and analyzed, and the principal component analysis was used to comprehensively evaluate the ability of each table grape variety. [Results] (1) The photosynthetically active radiation of greenhouse in different positions in general shows that before the shed>behind the shed>middle the shed, and the overall performance in different trellis in general shows that on the trellis＞middle the trellis＞under the trellis. (2) The SPAD values of grape leaves on different trellis was shown as on the trellis＞middle the trellis＞under the trellis, and the order from high to low among varieties was ‘Crimson Seedless’‘Summer Black’‘Xinyu’‘Hutai 8’‘Sunshine Muscat’‘Queen Nina’.(3) The diurnal variation of Pn、Gs and Tr of each variety showed a bimodal curve, and the diurnal variation of Ci showed a U-shaped and W-shaped variation.Fv/Fo and Fv/Fm generally showed a downward trend and then an upward trend. (4) The photosynthetic capacity of six grape varieties was ‘Crimson Seedless’>‘Summer Black’ >‘Sunshine Muscat’ >‘Xinyu’>‘Hutai 8’>‘Queen Nina’. [Conclusion] ‘Crimson Seedless’ and ‘Summer Black’ have higher Pn、Gs、Tr、Fo、Fm and lower Fv/Fo and Fv/Fm compared to other varieties, and are better adapted to the Hotan area, able to adapt to the local environment of high temperature and highlight intensity facilities.