[Objective] In order to provide data support and theoretical guidance for grassland restoration and biodiversity protection, the effects of grazing suspension in spring on the niche of Carex tibetikobresia meadow plants were explored. [Methods] Taking different grazing time as the environmental gradient, the importance value, aboveground biomass, niche breadth and overlap value of different economic groups of typical Carex tibetikobresia meadow in three-river source region were analyzed by using single factor analysis of variance and r software package (Pheatmap). [Results] Grazing for 50 days increased the important value of Cyperaceae (15.09%) and decreased the important value of poisonous weeds (50.09%);The aboveground biomass of different economic groups (except poisonous weeds) was significantly increased by 50 days, which increased by 220.99%, 69.73% and 139.37% respectively; Carex tibetikobresia S. R. Zhang、Helictotrichon tibeticum(Roshev) Holub(0.999); There are 55 pairs with (NO) greater than 0.990, accounting for 26.10% of the total, and 3 pairs with NO less than 0.75, accounting for 1.43% of the total.[Conclusion]The grazing moratorium in spring affected the important values and aboveground biomass of different economic groups in Carex tibetikobresia meadow. From the niche point of view, grazing suspension in spring is beneficial to the growth of fine forage and can effectively promote grassland restoration.