【Objective】 In order to explore the role of TaRING1 in the interaction between wheat and stripe rust, analyze its mechanism and provide theoretical basis for the green prevention and control of wheat stripe rust.【Methods】 The interaction target protein of TaRING1 was screened by yeast two-hybrid technique and verified by bimolecular fluorescence complementation and luciferase complementation assays. The ubiquitin function of TaRING1 was verified by ubiquitination experiments. The subcellular localization of target TaRIP92 was observed by transient expression in Nicotiana benthamiana and protoplast transformation in wheat. 【Results】 Using TaRING1 as bait, the interaction target TaRIP92 was screened by yeast two-hybrid technology, and the interaction between TaRING1 and TaRIP92 was verified by LCA and BiFC. In vitro ubiquitination experiments proved that TaRING1 could ubiquitinate TaRIP92. Transient expression of Nicotiana benthamiana and wheat protoplast transformation showed that TaRIP92 protein was localized in mitochondria. 【Conclusion】 TaRING1 interacts with mitochondrial protein TaRIP92 and ubiquitinates it.