[Objective] Tuber mustard has high economic value. Investigating the function of jasmonic acid signaling pathway gene JAZ8 in plant response to salt stress can provide genetic resources for breeding resistant varieties of tuber mustard. [Methods] The gene structure, promoter cis-acting elements and the physical and chemical properties of JAZ8 were analyzed by bioinformatics method. JAZ8 gene was cloned by molecular biology and overexpression vector was constructed. The JAZ8 gene of tuber mustard was overexpressed in the model plant Arabidopsis Thaliana by transgenic technology and the specific function of JAZ8 gene in Arabidopsis thaliana in response to salt stress was analyzed. [Results] The JAZ8 gene coding sequence was 396 bp, encoding 131 amino acids, containing TIFY and CCT-2 domains. The gene promoter contains cis-acting elements that respond to abscisic acid, salicylic acid and participate in defense and stress response. The phenotypic analysis of transgenic plants showed that Arabidopsis plants overexpressing BjuJAZ8 showed a more sensitive phenotype to salt stress during germination and greening. [Conclusion] Overexpression of JAZ8 in tuber mustard enhanced the sensitivity of Arabidopsis to salt stress.
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CAI Zhaoming, CHENG Chunhong, YU Hong, WANG Diandong. Overexpression of BjuJAZ8 in tuber mustard enhanced the sensitivity of Arabidopsis thaliana to salt stress[J]. Acta Botanica Boreali-Occidentalia Sinica,2024,44(11):1743-1751