【Objective】TIFY transcription factors are involved in the regulation of growth and development processes in plants. This experiment involves the identification of the members of the TIFY family in Luffa cylindrica, selecting the highly expressed members in the fruit, and further cloning and investigating their expression patterns in different tissues, thus providing a basis for the functional identification of these genes. 【Methods】Bioinformatics methods were used to identify members of the TIFY gene family in L. cylindrica. Transcriptome analysis was performed to screen for highly expressed members in L. cylindrica fruit, followed by cloning using RT-PCR. Tissue expression was analyzed using qRT-PCR. 【Results】 The L. cylindrica genome contains 17 TIFY genes, unevenly distributed across 9 chromosomes, with CDS lengths ranging from 297 bp to 1 353 bp, classified into four subfamilies. Transcriptome analysis revealed that LcTIFY2, LcTIFY7, LcTIFY11, and LcTIFY13 are significantly more highly expressed in L. cylindrica fruit compared to other members. The CDS sequences of these genes were cloned, with structures comprising 3, 5, 6, and 2 exons, respectively. Tissue expression analysis indicated that all four genes have the highest expression in the peel of L. cylindrica fruit. Additionally, LcTIFY7 and LcTIFY13 showed significantly higher expression in reproductive organs than in vegetative organs, while LcTIFY11 exhibited significantly higher expression in female flower buds and ovaries than in male flower buds. 【Conclusion】The TIFY family members LcTIFY2, LcTIFY7, LcTIFY11 and LcTIFY13 may play roles in reproductive growth and fruit development of L. cylindrica.