ZHAO Xiao-ying~ , REN Ji-zhou~ , WANG Yan-rong~ , LI Yan-mei~
2005, 25(2).
Abstract:对本地种白毛锦鸡儿(Caragana licentiana)和甘蒙锦鸡儿(Caragana opulens)以及外来种中间锦鸡儿(Caragana intermedia)种子进行了室内和野外萌发试验。结果表明,白毛锦鸡儿种子最适萌发温度为20℃,5℃时种子不萌发,甘蒙锦鸡儿最适萌发温度为10-20℃,在5℃和30℃时萌发率显著低于其它温度下的萌发率,中间锦鸡儿种子最适萌发温度为15-30℃,5℃下的萌发率为8.5%。室内萌发试验和野外萌发试验都表明白毛锦鸡儿种子萌发的速度缓慢,这体现在达到最大萌发率需要的时间长,萌发速度慢,且有4.0%的硬实种子,甘蒙锦鸡儿种子萌发的速度快,无硬实种子,高温下萌发受到一定的抑制。中间锦鸡儿种子在高温下萌发率高,速度快。启运白毛锦鸡儿种子萌发的最低土壤含水量为1.25%,甘蒙锦鸡儿和中间锦鸡儿的分别为3.75%和2.50%。白毛锦鸡儿种子萌发需要的土壤含水量低,种子萌发慢,且有小部分硬实种子,这可能是白毛锦鸡儿适应特殊环境的对策之一。
PENG Tao , WANG Wei , CUI De-cai , LI De-quan~
2005, 25(2).
Abstract:Wheat cultivars,CHL5 and BN2(drought-resistant ),LM5 and 921842 (drought-sensitive),were used in the study. Different concentrations of PEG-6000 were used to induce different osmotic stress conditions. The activity of amylase, content of soluble sugar, os
ZHANG Shao-ling , CHEN Di-xin , KANG Lang , WANG Ling
2005, 25(2).
Abstract:采用花粉液体培养法研究不同培养基组分和pH值对梨花粉萌发和花粉管生长影响.结果表明:培养基内硼酸、氯化钙、PEG-4000、蔗糖、葡萄糖、麦芽糖、山梨醇、果糖在一定浓度范围内,对花粉萌发及花粉管生长起促进作用,但超过一定浓度时起抑制作用;最适的培养基组分为:30mmol/LMES、0.01%硼酸、0.03%CaCl2·2H2O、15%PEG-4000、5%果糖 或5%山梨醇、10%蔗糖 ,最适的pH值为6.5.在该培养基内梨花粉萌发率约为59.2%,花粉管生长长度为966.3μm.
YUE Xiang-guo , HAN Fa~ , SHI Sheng-bo , LI Hui-mei
2005, 25(2).
Abstract:The leaves of Gentiana straminea that grows in greenhouse were treated with three differently complementary UV-B,and then their net photosynthetic rate, apparent quanta efficiency and dark respiration.The measarements showed that the three different UV-B
2005, 25(2).
Abstract:对野生风干发菜水分生理指标进行了测定与分析,结果表明:风干含水量为7.3%~11.2%,饱和含水量为616.0%~1258.4%,相对含水量1%左右,水分饱和亏为99%左右,水势为-8.2MPa;最大吸水速率为8.029gH2O·g-1·min-1,饱和吸水后其体积膨大10~14倍,平均吸胀率为1286.9%,其中伸长率为23.5%,增粗率为235.0%;导水力极弱;干燥发菜具有超强的吸水力,能在相对湿度>28.4%的空气中吸取水分;发菜的保水力随环境而变,在阴暗或弱光照的环境中具有较强的保水力.另外,探讨了不同种源发菜的水分生理指标与环境因子的关系.
2005, 25(2).
Abstract:盆栽和人工光源条件下,玉米叶片在普通空气中对强光照射不敏感,在高浓度CO2中强光照射0~5h后,光合速率(Pn)逐渐降低,无机磷(Pi)限制是其主要原因之一;大豆叶片在普通空气中受强光照射5h后,Fv/Fm、Pn、羧化效率(CE)和表观量子效率(AOY)明显降低,Fo升高,在高浓度CO2和强光下大豆Fo上升、Fv/Fm和气体交换参数下降的幅度减小。研究表明,高浓度CO2可减轻强光对植物尤其是C3植物光合功能的损伤,有限地缓解光抑制,但不能完全消除强光导致的大豆Pn和气孔导度(Gs)的降低。
WEI Hui-min , CHEN Yun-wei , ZHANG Nian-hui , ZHAO Yun , DU Lin-fang~
2005, 25(2).
Abstract:From 10-day old rapeseed (Brassica napus L.) mutant type Cr3529 and its wild type 3529, the cotyledon thylakoid membranes were respectively prepared and a comparative study on their chlorophyll composition, room temperature absorption spectra, chlorophyl
WANG Rui-qing , MA Shu-shang~ , WU Chun-lin , WANG Zhao-gai , TANG Yan
2005, 25(2).
Abstract:以'嘎拉'('Kid's Orange'×'Delicious')苹果为试材,研究了0℃贮藏期间及贮藏30、60、90和120 d后转入室温7d货架期间1-MCP(1-甲基环丙烯)对果实呼吸速率、乙烯释放速率、品质和蛋白质变化的影响.结果表明,1-MCP处理显著抑制嘎拉苹果贮藏期间和贮后货架期间呼吸和乙烯释放速率,延缓果实硬度、可滴定酸含量的下降,对可溶性固形物含量无影响.对照果实在贮藏过程中,出现5条明显的特异性蛋白条带,1-MCP处理能抑制特异蛋白表达.300 nL·L-1浓度1-MCP处理与600 nL·L-11-MCP处理作用效果无显著差异.
HUANG Yong-hong~ , TAO Xing-lin~ , LU Lu~
2005, 25(2).
Abstract:A donator plasmid pBI-aACO1 was digested with restriction endonucleases, then the acquired target genes was ligated to recipient plasmid pCAMBIA2301 which was digested with the same restriction endonucleases, and finally a plant expression vector named pC
2005, 25(2).
Abstract:The bulked Segregant Analysis (BSA) was used to examine and evaluate the feasibility and the efficiency of the identification of markers linked to resistance to bacterial wilt (Ralstonia solanacearum) in potato. A highly resistant primitive cultivated spe
2005, 25(2).
Abstract:用不同剂量60Coγ对以枸杞叶为外植体诱导出的愈伤组织进行辐射处理,并将恢复增殖的愈伤组织采用逐步提高盐浓度的方法,直到筛选出耐1%的愈伤组织变异体,通过对其生理生化的分析表明:变异体在不同盐浓度胁迫下干、鲜重增长均高于对照组;MDA含量和质膜透性、O-·2与H2O2含量都低于对照组;抗氧化酶SOD、POD、CAT活性均高于对照组;对可溶性蛋白SDS电泳结果表明:耐盐变异体有22条蛋白带,而对照组有21条蛋白带,其中31.3kD、21.0kD、18.5kD带为耐盐变异体所特有,而74.1kD、52.1kD带为对照组所特有,并且73.7kD、67.9kD的蛋白耐盐变异体含量高于对照组.
LIU Fei-hu~ , LIANG Xue-ni~ , HE Han~ , XIONG Li~
2005, 25(2).
Abstract:采用LI-6400便携式光合仪对室外栽培的几种野生银莲花光合作用特性进行了研究.结果表明,净光合速率 Pn 和蒸腾速率 Tr 日变化均呈单峰曲线,前者的峰值出现在10:00~11:00时,后者的峰值出现在13:00时前后.在光强0~2000μmol·m-2·s-1条件下,Pn呈S曲线,光补偿点为60~80μmol·m-2·s-1,光饱和点为800μmol·m-2·s-1左右,但光强继续增加到1800μmol·m-2·s-1,Pn仍有少许提高;Tr随PAR的增加而缓慢增加.在环境CO2浓度为0~350μmol·mol-1条件下,Pn直线上升,草玉梅、秋牡丹和野棉花的光合CO2补偿点均为50μmol·mol-1左右;Tr在环境CO2浓度25~350μmol·mol-1范围内几乎呈水平线.野生银莲花的Pn和Tr表现出较明显的种间差异.
2005, 25(2).
Abstract:白首乌叶片光合日变化曲线呈明显双峰型,峰值分别出现在上午9:00~10:00时和下午3:00时左右。在高光强下中午有明显“午休”现象。叶绿素荧光参数Fv/Fm和φPSⅡ在晴天中午明显降低,说明田问白首乌叶片在晴天中午经常发生光抑制,高光强和高温是发生光抑制的主要原因。
2005, 25(2).
Abstract:Two-year-old young trees of 'Guoqing No.4', satsuma mandarin whose rootstocks were red tangerine +trifoliate and red tangerine +rough lemon somatic hybrid, Troyer citrange and Swingle citrumelo sexual hybrid, trifoliate(CK)were used to study on the effect
GAO Xiang , TONG Sheng-li , ZHANG Gai-sheng
2005, 25(2).
Abstract:The relationship between protein contents, sedimentation values and high molecular weight (HMW) glutenin subunits in 150 wheat varieties were studied. The results showed that the subunits controlled by Glu-A1, Glu-B1 and Glu-D1 were closely related to qua
2005, 25(2).
Abstract:以 拉宾斯 、 斯坦勒 等7个生产上主栽的欧洲甜樱桃品种为试验材料,研究了不同品种采收后果实可溶性蛋白质、维生素C Vc 、可溶性总糖和总酸等主要内含物含量的差异.进一步分析了各主要内含物含量与果实发育期、果实大小以及相互之间的相关性.结果表明:甜樱桃品种果实可溶性蛋白质和总糖含量与果实发育期均呈显著正相关,说明发育期短的早熟品种果实可溶性蛋白质和糖含量显著低于果实发育期长的中、晚熟品种 P<0.05 ;甜樱桃品质标准在 中上 级以上,果实的可溶性蛋白质含量范围在1052.0~1119.3mg·100g-1FW;品质在 中上 标准以上的果实维生素C的含量范围在9.89~18.01mg·100g-1FW;7个甜樱桃品种之间果实总酸度差异显著 P<0.05 ;甜樱桃果实的糖酸比值与果实发育期和可溶性糖含量均呈显著正相关,甜樱桃果实的糖酸比值能确切地反映果实风味及品质的优劣,可以作为一项指标,甜樱桃品质在 中上 标准以上的果实糖酸比范围在31.37~40.54;早、中、晚熟品种之间相比较,甜樱桃早熟品种的果实大小显著低于中、晚熟品种 P<0.05 .
WANG Shang-de , KANG Xiang-yang~ , Ministry of Education , Beijing Forestry University , China)
2005, 25(2).
Abstract:According to drupe size and taste of Nitraria tangutorum Bobr.,39 plus trees of fruit-use were selected as experimental materials with which the study about variations of fruiting-cyme characters was carried out. The results showed: there was distinct dif
ZHANG Li-ming~ , WANG Qing-mei~ , MA Dai-fu~ , WANG Yi~
2005, 25(2).
Abstract:1 580 entries of sweetpotato samples from three Provinces ,Shandong, Jiangsu and Anhuai were tested with ELISA method. The result showed that SPFMV and SPLV were the two popular viruses in Huang-huai sweetpotato production region of China which the infect
2005, 25(2).
Abstract:The characteristics of species diversity of four typical manmade communities in forest region of Mountain Wutai, Shanxi province was systematically studied. The results were as follow:Firstly, the differences of Simpson index and Shannon-Wienner index of
2005, 25(2).
Abstract:The paper aims to explore the contribution of soil nutrients to vegetation restoration.According to factor analysis of soil nutrients during vegetation restoration in abandoned lands in hilly and gully regions of the Loess Plateau,the effect of the soil n
2005, 25(2).
Abstract:通过对高寒嵩草草甸土壤剖面不同深度(0~5cm,5~15cm,15~25cm,25~35cm,35~50cm,50~65cm)有机碳稳定性碳同位素的测定发现,土壤有机碳稳定性同位素(δ^13C)随土壤深度的增加而变大。表层土壤(0~5cm,定义为现代土壤)的δ^13C值最小,基本上接近现代植被的碳同位素特征。在土层5~10cm深度以下(粗略地定为古土壤),土壤有机碳稳定性同位素骤然上升,与表层土壤的同位素特征明显不同。考虑到影响土壤碳同位素的诸多因素,通过稳定性碳同位素的质量平衡模型计算,得出初步结果:来自C4(或CAM)植物的碳源随土壤深度的增加而增大。进一步推测,该地区植被可能经历由C4植物占优势的群落向C3植物占优势的群溶演化的过程。在这个过程中,大气碳同位素的变化和土壤有机质的形成过程(有机质淋溶过程)等也会引起土壤碳同位素的升高,因此质量平衡模型可能会过多地估算C4组分,而低估C3组分。
TANG Yuan-jiang , XIE Zhong-yu , LIAO Jing-ping~ , WU Qi-gen
2005, 25(2).
Abstract:研究了姜群4科代表植物的种子解剖学和组织化学,结果表明,姜科、闭鞘姜科和竹芋科均具假种皮。姜科的外种皮由1层细胞壁增厚的表皮细胞构成,中种皮分化为下皮层、半透明细胞层和色素层,内种皮由石细胞构成;闭鞘姜科外种皮由薄壁细胞构成,中种皮为薄壁细胞层,内种皮由石细胞构成;美人蕉科外种皮则由径向延长的Malpighian细胞构成,中种皮包含4~5层石细胞和2~5层薄壁细胞,内种皮则由色素细胞组成;竹芋科外种皮和中种皮均由薄壁细胞构成,内种皮由1层垂周壁和内平周壁极其增厚的石细胞构成。姜群中只有美人蕉科不具假种皮和孔盖,姜科和闭鞘姜科的合点区均有色素细胞群,闭鞘姜科的合点区还分化出一个内方有缺口的合点室。姜科和闭鞘姜科都有明显的内胚乳和外胚乳;美人蕉科具发达的外胚乳,内胚乳仅为一薄层;竹芋科种子不具内胚乳。综合芭蕉群种子解剖学和组织化学的研究结果,讨论了姜群4科之间的系统学关系。
2005, 25(2).
Abstract:借助显微和扫描电镜技术对石蒜属植物叶表皮微形态特征进行了研究。利用光学显微镜和扫描电子显微镜观察了石蒜属植物的叶表皮,统计并测量了气孔类型、气孔大小、气孔密度及气孔指数等,描述了气孔及气孔外拱盖的有关特征。结果表明:石蒜属植物叶表皮气孔器为无规则型,近轴面表皮细胞形状、气孔器类型、垂周壁式样、气孔大小及气孔外拱盖内缘种间无差异或极小,表明石蒜属植物为一自然分类群。而远轴面表皮细胞形状、气孔密度、气孔指数、气孔是否下陷、气孔外拱盖是否有蜡质纹饰等种间差异较大。根据远轴面叶表皮细胞形状及叶气孔特征,研究表明:换锦花、长筒石蒜和安徽石蒜,夏水仙、乳白石蒜与红蓝石蒜,石蒜与中国石蒜具有较近的亲缘关系。因此,叶微形态特征对探讨石蒜属植物种间亲缘关系具有一定的意义。
2005, 25(2).
Abstract:On the basis of the culture of Bryum uliginosum spores, the processes of spore germination, protonema development and gametophyte were observed. The results showed that spores germinated breaking the exospore and producing chloronemata directly, while cau
ZHANG Ying , ZHOU Yong-hong~ , ZHANG Li , ZHANG Hai-qin , YANG Rui-wu , DING Chun-bang
2005, 25(2).
Abstract:RAMP analysis of 23 species of Roegneria,Elymus,Hystrix and Kengyilia showed that RAMP variation among the four genera was extremly large. 31 primers combinations produced 286 bands, which were all polymorphic. The materials of Roegneria,Elymus,Hystrix an
FU Da-xu~ , ZHANG Hui~ , SU Xue~ , CHEN Xue-lin~ , SUN Kun~
2005, 25(2).
Abstract:The random amplified polymorphic DNA (RAPD) was uesed to examine 11 populations of Morus alba Linn. and Morus nigra Linn. cultivated in Xinjiang. 87.39% total scorable markers were polymorphic and Shannon's Information index (I) was 0.399 7 at genus level
2005, 25(2).
Abstract:In this study the fruiting body of basidiomycete Scleroderma citrinum was investigated for its insecticidal property against 3-instar Mythimna separate. At the concentration of 25.0 mg/mL, the antifeeding rates of chloroform-methanol total extract of S.
ZHANG Cai-xia , LI Yu-lin , HU Feng-zu~
2005, 25(2).
Abstract:利用硅胶柱层析分离和Sephadex LH20纯化等方法从珠芽蓼果实分离得到6个化合物,经^1H NMR、^13C NMR等技术及理化性质鉴定为β-谷甾醇、胡萝卜苷、没食子酸、正丁基—β—D吡喃果糖苷、槲皮素—5—O—β—D—葡萄糖苷、蔗糖。6种化合物均为首次从该植物分离得到。
2005, 25(2).
Abstract:Histochemical technique and phytochemical methods were applied in the studies on the distribution and accumulation of ginsenosides in the tissues of Gynostemma pentaphyllum,a medical herb, whose buds, young stems and leaves were processed as Longxu tea. S
MA Yu-hua , JI Lan-ju~ , JI Wen-he , CHEN Gui-chen , LU Xue-feng
2005, 25(2).
Abstract:采用反相高效液相色谱法对川西獐牙菜中6种药用成分不同生长期的含量进行了测定.色谱柱为Kro-masilC18 250mm×4.60mm,5μm ,流动相为甲醇-0.02%的磷酸水溶液,检测波长260nm.该方法具有很好的线性关系和回收率.结果显示,川西獐牙菜全草的最佳采收期为9月中旬 花果期 .
2005, 25(2).
Abstract:建立测定了岩白菜中岩白菜素含量的方法.色谱柱:WatersC18柱 5μm×3.9mm×150mm ,流动相:甲醇∶水∶磷酸=20∶80∶0.1,流速为1.0mL/min,检测波长275nm,AUFS0.01,柱温为室温.结果表明,岩白菜素在0.16~0.08μg有良好线性关系,r=0.9992,平均回收率为98.14%,RSD为1.12%.本方法是测定岩白菜中岩白菜素含量的快速、简便、准确可靠的定量方法.
DU Bao-guo , YANG Tu-xi , WEI An-zhi , YANG Feng-li
2005, 25(2).
Abstract:In order to study the best method for tissue culture and rapid propagation for Amelanchier alnifolia Nutt., stems with buds were chosen as explants. The results of in vitro culture showed that the shoot differentiation medium was MS+IAA 0.1 mg/L+6-BA 0.5
2005, 25(2).
Abstract:Metabolome is the offspring of genome, transcriptome and protome, through qualitative and quantitative analysis of metabolites to define the profile of cell or organ, then to explain the express process of functional gene. Metabolome is reviewed in the ar
ZHAO Li-ying , DENG Xi-ping , SHAN Lun
2005, 25(2).
Abstract:Drought stress is a bottleneck factor for plant growth and development. Water stress induce the increased generation of active oxygen species, which cause oxidative stress to plant cells. some are highly toxic and rapidly detoxified throughvarious cellula
LI Hong-fang~ , TIAN Xian-hua~ , REN Yi~
2005, 25(2).
Abstract:The research progress in the vessels and perforation plates of the vascular plants, including characters of vessels (including the type of perforation plates, multiple and lateral-wall perforation plates, pit membrane remnants), the origin of the vessels